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recording chamber  (Warner Instruments)

 
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    Warner Instruments recording chamber
    Recording Chamber, supplied by Warner Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recording chamber/product/Warner Instruments
    Average 86 stars, based on 1 article reviews
    recording chamber - by Bioz Stars, 2026-05
    86/100 stars

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    A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using <t>Daniovision</t> for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.
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    A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using Daniovision for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.

    Journal: bioRxiv

    Article Title: In vivo discovery of blood-brain barrier opening small molecules with FishNAP

    doi: 10.64898/2026.03.18.712473

    Figure Lengend Snippet: A . Schematic of the FishNAP assay, with up to seven larvae per well. Overall activity is monitored using Daniovision for 1 hour after loperamide exposure; increased BBB permeability leads to reduced locomotor activity. B . Leaky 3 dpf larvae are significantly sedated compared to 5 and 7 dpf fish, as previously reported (O’Brown, 2019). C . spock1 mutants show significantly reduced activity compared to wild-type (WT) controls at 7 dpf using FishNAP. D . Workflow schematic for assessing BBB permeability in cldn5a crispant fish. E–H . Tracer leakage assays using 1 kDa NHS ester (E, turquoise) and 10 kDa Dextran (F, gold) reveal significantly increased 1 kDa NHS tracer permeability outside of the kdrl:mCherry + vasculature (magenta) in cldn5a crispants vs. uninjected controls (G), but no significant change in 10 kDa Dextran permeability (H). I . FishNAP also detects increased BBB permeability in cldn5a crispants relative to uninjected controls. The slowest fish (red outlines) show greater than 95% cldn5a knockdown. ** p<0.01, *** p<0.001, **** p<0.0001 by one-way ANOVA (B and C) and Welches t-test (G-I). Scale bar represents 50 µm.

    Article Snippet: The plate was then placed in a Daniovision recording chamber (Noldus).

    Techniques: Activity Assay, Permeability, Knockdown